class: title-slide, middle, left ## Session 7: Recap of how to perform quality control of your data ### Dr Shazia Ruybal-Pesántez Presented at the [covidClassifyR Shiny app workshop](https://shaziaruybal.github.io/covidClassifyR-workshop) for researchers from PNGIMR and partner institutions 2022-03-17 --- class: left # Recap -- ####
**[Session 1](https://shaziaruybal.github.io/covidClassifyR-workshop/sessions/2022-03-10-session1/index.html)** gave you an overview of the Luminex technology and the COVID-19 multi-antigen serological assay that was established in PNG -- ####
**Session 2** gave you an overview of the purpose of serosurveillance and insights into the COVID-19 sero-surveys that have been carried out in PNG -- ####
**[Session 3](https://shaziaruybal.github.io/covidClassifyR-workshop/sessions/2022-03-10-session3/index.html)** gave you an overview of the [`covidClassifyR`](https://shaziaruybal.shinyapps.io/covidclassifyr) Shiny app -- ####
**[Session 4](https://shaziaruybal.github.io/covidClassifyR-workshop/sessions/2022-03-11-session4/index.html)** gave you an overview of how to prepare and import your data to the [`covidClassifyR`](https://shaziaruybal.shinyapps.io/covidclassifyr) Shiny app -- ####
**[Session 5](https://shaziaruybal.github.io/covidClassifyR-workshop/sessions/2022-03-11-session5/index.html)** gave you an overview of how to prepare your data and upload your data to the [`covidClassifyR`](https://shaziaruybal.shinyapps.io/covidclassifyr) app, and how to check the Luminex run info, raw data file and user-supplied plate layout -- ####
**[Session 6](https://shaziaruybal.github.io/covidClassifyR-workshop/sessions/2022-03-11-session6/index.html)** gave you an overview of how to download your processed data and quality control report -- .footnote[ For all the workshop materials see the [workshop website
](https://shaziaruybal.github.io/covidClassifyR-workshop/materials.html) ] --- class: center #
# Today we will cover: -- ### A brief recap of how to perform quality control of your data -- ### How to download your processed data and QC report -- ### You can find the app [here
](https://shaziaruybal.shinyapps.io/covidClassifyR) --- #
Feedback -- ###
Did you download the example data and practice importing it to the [covidClassifyR
](https://shaziaruybal.shinyapps.io/covidClassifyR)? -- ###
Did you go through the quality control section of the [covidClassifyR
](https://shaziaruybal.shinyapps.io/covidClassifyR)? -- ###
Did you download the example output data [covidClassifyR
](https://shaziaruybal.shinyapps.io/covidClassifyR)? --- #
Quality control of your data: recap -- ####
What do you expect your standard curves to look like? -- <img src="img/std_curves.png" width="900" height="500" /> --- #
Quality control of your data: recap -- ####
What do you expect your bead counts to be to indicate a "good run"? -- <img src="img/plate_qc.png" width="800" height="400" /> --- #
Quality control of your data: recap -- ####
What MFI do you expect your blank samples to be at to indicate a "good run"? -- <img src="img/blanks.png" width="800" height="400" /> --- #
Quality control of your data: recap -- ####
What kind of model do we use to convert our data from MFI to RAU? -- <img src="img/model.png" width="800" height="400" /> --- #
Quality control of your data: recap -- ####
What do we expect in our processed data? -- <img src="img/sample_results.png" width="900" height="400" /> --- ##
Downloading your data: recap -- ![](img/download_qc.png) -- ### _
What do we download if we click on "Download MFI/RAU data"?_ --- background-image: url(img/eg_MFIRAU.png) background-position: bottom center background-size: 75% ###
Processed MFI/RAU data output --- ##
Downloading your data: recap ![](img/download_qc.png) -- ### _
What do we download if we click on "Download standard curve data"_ --- background-image: url(img/eg_std.png) background-position: middle center background-size: 75% ###
Standard curve data output --- ##
Downloading your data: recap ![](img/download_qc.png) -- ### _
What do we download if we click on "Download quality control report"_ --- background-image: url(img/eg_report.png) background-position: right center background-size: 75% .left-column[ ###
Quality control report output ] --- class: inverse, middle, center #
## Next session: ### Introduction to the classification algorithm --- # Acknowledgments - Dr Maria Ome-Kaius and Dr Fiona Angrisano - PNGIMR and partner institutions - WEHI & Burnet Institute - All of you for attending! *We are extremely grateful for financial support to develop and host the covidClassifyR Shiny web application, and to host these virtual workshops through the [Regional Collaborations Programme COVID-19 Digital Grant](https://www.science.org.au/news-and-events/news-and-media-releases/regional-research-set-get-digital-boost) from the Australian Academy of Science and Australian Department of Industry, Science, Energy and Resources.* The scripts and functions used in [`covidClassifyR`](https://shaziaruybal.shinyapps.io/covidclassifyr) were developed by Shazia Ruybal-Pesántez, with contributions from the following researchers: Eamon Conway, Connie Li Wan Suen, Narimane Nekkab and Michael White. .footnote[ _These slides were created using the R packages: [xaringan](https://github.com/yihui/xaringan), [xaringanthemer](https://github.com/gadenbuie/xaringanthemer), [xaringanExtra](https://github.com/gadenbuie/xaringanExtra)_ ] --- name: contact class: inverse .pull-left[ .center[ ### Dr Shazia Ruybal-Pesántez <img style="border-radius: 50%;" src="https://shaziaruybal.github.io/covidClassifyR-workshop/images/shazia.png" width="250px"/> #### Contact details [
ruybal.s@wehi.edu.au](mailto:ruybal.s@wehi.edu.au) [
@DrShaziaRuybal](https://twitter.com/DrShaziaRuybal) ]] .pull-right[ ### Session 7 Resources: ### [
Recording](https://youtu.be/OB1tH3r2MS4) ### [
`covidClassifyR`](https://shaziaruybal.shinyapps.io/covidClassifyR) ### [
Workshop materials](https://shaziaruybal.github.io/covidClassifyR-workshop/materials.html) ### [
Slides for Session 7](https://shaziaruybal.github.io/covidClassifyR-workshop/slides/session7/session7_slides.html) ] --- class: inverse, middle, center #
Questions?